|Season 1, Episode Special|
- Mickey Mouse- Entertainment superstar and general manager/co-owner of the House of Mouse. Mickey is most visible as the club's flamboyant theater host and, in practice, leaves most of the club's management to the other staff.
- Minnie Mouse- Mickey's girlfriend, show planner and bookkeeper. Minnie is responsible for much of the club's day-to-day administration. Her cool, rational personality has kept the club running in times of crisis.
- Donald Duck- Mickey's best friend and assistant manager/co-owner of the House of Mouse. Donald is responsible for the overall customer service at the club and attends to the needs of the club's VIP guests. He secretly covets Mickey's fame and position at the club.
- Daisy Duck- Donald's girlfriend and reservation clerk. Somewhat frivolous, Daisy harbors ambitions to perform on-stage at the club with various acts.
- Goofy- Mickey and Donald's friend, Max's father, and head waiter of the club. Clumsy yet lovable, Goofy manages the club's restaurant operations.
- Pluto- Mickey's pet dog and club mascot.
- Horace Horsecollar- Mickey's long-time friend and technician. Horace runs and maintains the club's technical equipment including lights, loudspeakers, and video players.
- Clarabelle Cow- Minnie's long-time friend and gossip columnist. Clarabelle collects gossip and rumors from all over Toontown for her talk show at the club. Her gossip has occasionally lead to problems for the club's staff.
- Max Goof- Goofy's son and valet parking attendant.
- Huey, Dewey, and Louie- Donald's three nephews and a house band with alternating names such as "The Quackstreet Boys", "Quackwork", "Kid Duck" and "The Splashing Pumpkins".
- Gus Goose- Donald's cousin and executive chef of the club. A notorious glutton, Gus sometimes eats the food he prepares for the guests just before it is served.
- Magic Mirror- Magical artifact and on-site consultant. Mickey and his friends sometimes ask for advice from the Magic Mirror on a variety of issues. He is also available for queries from the club's guests. Unsurprisingly, the Evil Queen can sometimes be found staring at the Mirror.
- Mike the Microphone- A talking microphone and announcer. He usually assists Mickey in presenting and ending events on-stage nightly.
- Penguin waiters- Waiting staff of the club. According to an advertisement at the club, they are also available for hire on a temporary basis.
- Animated brooms- Janitorial staff of the club.
- Pete- The club's greedy landlord, who often attempts to shut the theater down for his own personal gain by sabotaging the show, since Mickey's contract states that the club stays open only for as long as the show goes on.
|1||"The Stolen Cartoons "||January 13, 2001||
In an attempt to end the show and shut down the House of Mouse, Pete steals all the cartoons. Meanwhile, Donald is put in charge of the club after Mickey and Goofy leave to film a new cartoon. Pete is eventually found out when he tries to point out the new cartoon is the only one they have, accidentally letting the others fall out of his coat.
Cartoons: Pluto Gets the Paper: Wet Cement (2001), Donald's Dynamite: Magic Act (2000), Hickory Dickory Mickey (2001)
|2||"Big Bad Wolf Daddy "||January 27, 2001||
Donald hires the Big Bad Wolf and the Three Little Pigs to perform a show at the club, which Mickey fears since the wolf damaged the club the last time he performed. The Three Pigs confront the Wolf at the end for blowing everyone away. However the doors fall onto the pigs, forming a house. This causes the wolf to blow the house, including the club, down.
Cartoons: Donald's Charmed Date (2001), Pluto Gets the Paper: Mortimer (2000), How to Be Groovy, Cool, and Fly (2001)
|3||"The Three Caballeros "||February 3, 2001||
The Three Caballeros are scheduled to perform at the club, but no one remembers that Donald was a Caballero. Determined to not be forgotten, he tries to be the most important performer of the group, re-naming himself "The Duck Formerly Known as Donald" (an obvious parody of Prince).
Cartoons: Donald's Fish Fry (2000), How to Be Smart (2001)
|4||"Goofy's Valentine Date "||February 10, 2001||
Cartoons: Donald's Valentine Dollar (1999), Mickey to the Rescue: Staircase (1999), Pluto's Arrow Error (1999)
|5||"Timon_and_Pumbaa"||February 17, 2001||
Timon and Pumbaa are set to perform, but end their friendship after they argue over what they are going to do in their act. Now it's up to Mickey and Donald to bring them back together.
Cartoons: Pluto's Magic Paws (2000), Mickey to the Rescue: Cage and Cannons (1999), Golf Nut Donald (2001)
|6||"Jiminy Cricket"||February 24, 2001||
Jiminy Cricket quits his job as Pinocchio's conscience after Pinocchio befriends Pain and Panic and decides to become Mickey's conscience. Mickey quickly realizes that Pinocchio and Jiminy must stay together and has to figure out how to do it.
Cartoons: Mickey's Mistake (1999), Daisy's Road Trip (1999)
|7||"Unplugged Club "||March 3, 2001||
Pete attempts to stop the show again by removing the electrical battery and turning off all of the club's electricity. Luckily, Mickey and Minnie turns it back on again with Zeus' lightning bolt.
Cartoons: Music Store Donald (2001), Mickey's Cabin (2000)
|8||"Gone Goofy "||March 10, 2001||
Donald tries to get Goofy fired from his job as head waiter after the club's budget causes problems.
Trivia: The Horned King makes an appearance in this episode.
Cartoons: Pit Crew (2001), Goofy's Extreme Sports: Shark Feeding (2000), Donald's Goofy World (2001)
|9||"Rent Day "||March 17, 2001||Before the show ends, Mickey needs to pay the rent for Pete after he unwittingly spends the money on cheese.
Cartoons: Mickey's Mountain (2000), Maestro Minnie: Circus Symphony (2001), Big House Mickey (2001)
|10||"Donald's Lamp Trade "||March 24, 2001||Jafar tricks Donald into thinking that Mickey is not his friend so he can steal a lamp for him.
Cartoons: Survival of the Woodchucks (2000), Goofy's Radio (2000)
|11||"Donald's Pumbaa Prank "||March 31, 2001||
It's April Fool's Day, and after Mickey plays a friendly prank on Donald, Pete takes advantage of Donald and convinces him to play a nasty prank that could shut down the club for good.
Cartoons: Mickey's April Fools (2001), Whitewater Donald (2000)
Note: When Pete Say's "Yeah And I Woulda Done It If Wasn't For That Meddling Duck" Is A Parody Of The Scooby Doo Saying: "Yeah And I Woulda Done It If Wasn't For Those Meddling Kids"
|12||"Thanks to Minnie "||November 8, 2004||Minnie feels that she is not appreciated at the House of Mouse after Clarabelle starts a rumor, so she quits her job, making the rest of the staff realize how much she means to them.
Cartoons: Minnie Visits Daisy (1999), Mickey's Big Break (2000)
|13||"Pluto Saves the Day "||November 29, 2004||Pluto has to save the club after Pete gives magical sleeping apples to the staff in another attempt to shut down the club.
Cartoons: Goofy's Big Kitty (1999), Pluto's Kittens (2000)